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Linkers, Spacers and PEGylation

Depending upon design and application we can insert a spacer/linker between the tags, protein(KLH,BSA) or fluorescent and the peptides. A common hydrophobic spacer is aminohexanoic acid (Ahx), and a common hydrophilic spacer is poly(ethylene) glycol (PEG). Others please reference the below listed table: such as Gly, AEA, Ava, ANP Linker, Beta-Alanine, C12, GABA, etc... PEGylated Peptides.

Peptide with PEG modification can improve their solubility, increase their half life in biological applications by protecting them from proteolytic digestion. In addition, PEGylation can mask the peptide from producing an immune response. Thus, pegylated peptide may overcome many of the challenges and are being examined as potential drugs and drug candidates.

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Name Length Structure
Beta-Alanine 4
4-Aminobutyric Acid (GABA) 5
(2-Aminoethoxy) acetic acid (AEA) 6
5-Aminovaleric Acid (Ava) 6
Aminohexanoic acid (Ahx) 7
12-amino-dodecanoic acid 13
3-Amino-3-(2-nitrophenyl)propanoic acid (ANP Linker) 4
Mini-PEG 9
PEG 4