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Fluorescence and Dye Labeling Peptides
Fluorescence and dye labeled peptides have been developed for in vivo biomedical imaging, protein binding and localization studies, etc. Fluorescence and dye can be attached to the peptide N-terminus directly (FITC always via be inserted an aminohexanoic acid (Ahx) or β-Ala linker between the fluorophore (fluoroscein) and the N-terminus of the peptide.), or to the Lys (or Cys) side chains in the peptide C-terminus or many other positions in the peptide.
ONTORES Peptides can provide many common used fluorescent and dyes peptides: such as FITC, FAM, TAMRA, NBD (7-nitrobenz-2-oxa-1, 3-diazole), Amc, Atto, Alexa Fluor, Bodipy, Cy3, Cy5......
Each fluorescence and dye labeled peptide delivered as a lyophilized powder in foil covered tube.
FITC
Fluorescein isothiocyanate (FITC) is an amine reactive derivative of fluorescein dye has been extensively used to label peptides and proteins.
Fluorescein or dye | Color | Absorption maximum | Emission maximum |
---|---|---|---|
FITC | Green | 495nm | 521nm |
FAM
FAM is the commonly used fluorescent dye in confocal laser-scanning microscopy and flow cytometry applications.

Fluorescein or dye | Color | Absorption maximum | Emission maximum |
---|---|---|---|
FAM | Green | 492nm | 517nm |
Rhodamine B
Rhodamine B represents one among a numerous range of rhodamine dyes, used in fluorescence assays.

Fluorescein or dye | Color | Absorption maximum | Emission maximum |
---|---|---|---|
Rhodamine B | Orange | 550nm | 580nm |
TAMRA
TAMRA is one of the most popular orange fluorophores used to label peptides and proteins.

Fluorescein or dye | Color | Absorption maximum | Emission maximum |
---|---|---|---|
FAM | Green | 544nm | 576nm |
NBD (7-nitrobenz-2-oxa-1, 3-diazole)
NBD-labeled peptides is reported as a popular choice in membrane and cell biological studies.

Fluorescein or dye | Color | Absorption maximum | Emission maximum |
---|---|---|---|
NBD | Green | 486nm | 543nm |
AMC
AMC (7-amino-4-methylcoumarinyl), UV-excitable dye, used in enzyme assays using cuvettes or flow cytometry.

Fluorescein or dye | Color | Absorption maximum | Emission maximum |
---|---|---|---|
AMC | Blue | 353nm | 422nm |
Cy3 and Cy5
Cy3 and Cy5 are dyes with extremely high extinction coefficients and fluorescence. Thus, they are especially suitable for very sensitive localisation assays of peptides in cells.Their disadvantage is the high instability of the molecules under peptide synthesis conditions.therefore the yields are comparatively low.
Fluorescein or dye | Color | Absorption maximum | Emission maximum |
---|---|---|---|
Cy3 | 558nm | 572nm | |
Cy5 | 646nm | 664nm |