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Fluorescence and Dye Labeling Peptides

Fluorescence and dye labeled peptides have been developed for in vivo biomedical imaging, protein binding and localization studies, etc. Fluorescence and dye can be attached to the peptide N-terminus directly (FITC always via be inserted an aminohexanoic acid (Ahx) or β-Ala linker between the fluorophore (fluoroscein) and the N-terminus of the peptide.), or to the Lys (or Cys) side chains in the peptide C-terminus or many other positions in the peptide.

ONTORES Peptides can provide many common used fluorescent and dyes peptides: such as FITC, FAM, TAMRA, NBD (7-nitrobenz-2-oxa-1, 3-diazole), Amc, Atto, Alexa Fluor, Bodipy, Cy3, Cy5......

Each fluorescence and dye labeled peptide delivered as a lyophilized powder in foil covered tube.

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FITC

Fluorescein isothiocyanate (FITC) is an amine reactive derivative of fluorescein dye has been extensively used to label peptides and proteins.

  • Labeled on the peptide N-terminus

  • Labeled on the lysine side chain

Fluorescein or dye Color Absorption maximum Emission maximum
FITC Green 495nm 521nm

FAM

FAM is the commonly used fluorescent dye in confocal laser-scanning microscopy and flow cytometry applications.

Fluorescein or dye Color Absorption maximum Emission maximum
FAM Green 492nm 517nm

Rhodamine B

Rhodamine B represents one among a numerous range of rhodamine dyes, used in fluorescence assays.

Fluorescein or dye Color Absorption maximum Emission maximum
Rhodamine B Orange 550nm 580nm

TAMRA

TAMRA is one of the most popular orange fluorophores used to label peptides and proteins.

Fluorescein or dye Color Absorption maximum Emission maximum
FAM Green 544nm 576nm

NBD (7-nitrobenz-2-oxa-1, 3-diazole)

NBD-labeled peptides is reported as a popular choice in membrane and cell biological studies.

Fluorescein or dye Color Absorption maximum Emission maximum
NBD Green 486nm 543nm

AMC

AMC (7-amino-4-methylcoumarinyl), UV-excitable dye, used in enzyme assays using cuvettes or flow cytometry.

Fluorescein or dye Color Absorption maximum Emission maximum
AMC Blue 353nm 422nm

Cy3 and Cy5

Cy3 and Cy5 are dyes with extremely high extinction coefficients and fluorescence. Thus, they are especially suitable for very sensitive localisation assays of peptides in cells.Their disadvantage is the high instability of the molecules under peptide synthesis conditions.therefore the yields are comparatively low.

Fluorescein or dye Color Absorption maximum Emission maximum
Cy3 558nm 572nm
Cy5 646nm 664nm