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Two New Bioactive Peptides

Published: 2016-12-26

Keyword:

Article from ourcustomer: Wang Xuan. China Animal Husbandry & Veterinary Medcine. 2015

 

Objectives: The study was aimed to research the growth inhibitory effect two new bioactive peptides Temporin-Lband Catesbeianin-1afrom Ranacatesbeianaon human lung cancer NCI-H446 cells, breast cancer MCF-7 cells and mice leukemia K562 cells, and provide the basis for selecting the new peptide antitumor drugs.

Results: The second structures of two new bioactive peptides were tested by circular dichroism spectrum(CD), and the effects of the two new bioactive peptides on human lung cancer NCI-H446 cells, breast cancer MCF-7 cells and mice leukemia K562 cells were examined with MTS cytotoxicity assay. The circular dichroism spectrum results showed the secondary structure of Temporin-Lb was PPII, and the secondary structure of Catesbeianin-1a was random coil. Using MTS cytotoxicity assay, it was found that given Catesbeianin-1a, the sharp of the three cancer cells above had little changed after culturing for 24 h, and the three cancer cells promoted normal. Gien Temporin-Lb, the cell morphology of three cancer cells had changed after culturing for 24 h, the growth of the three cancer cells above had been inhibited, especially the bioactive peptide Temporin-Lb had a sharp antitumor effect to mice leukemia K562 cells between the concentration of 4 and 40 μmol/L.

Conclusion: Bioactive peptide Catesbeianin-1a had no obvious effect on proliferation of the three cancer cells above. Bioactive peptide Temporin-Lb had a certain inhibitory effect to tumor cells.

 

Table 2 the effect of bioactive peptide Catesbeianin-1a on proliferation of tumor cells in vitro (n=5)

*,compared with the control group, it has a sifnificant difference(P<0.05).

Table 3 the effect of bioactive peptide Temporin-Lb on proliferation of tumor cells in vitro (n=5)

*,compared with the control group, it has a sifnificant difference(P<0.05).

 

Catesbeianlectin and Temporin-Lbwere synthesized according to the standard solid phase peptide synthesis by ZheJiang Ontores Biotechnologies Co. Ltd. (Hangzhou, China). It was desalted and purified by the HPLC (waters 600 controller) reversed phase C18 column chromatography. The relative molecular mass was determined by the fast atom bombardment mass spectrometry (AB3200). The purity was ≥95%.